Stem bark of Ziziphus xylopyrus, (Retz) Willd. was collected dried, powdered and subjected for extraction. Preliminary phytochemical screening showed the presence of alkaloids, carbohydrates, steroids and sterol, glycosides, saponins, flavonoids, phenolic compounds, triterpenoid in ethanolic and aqueous extracts of stem bark of Ziziphus xylopyrus, (Retz) Willd. Fractions were prepared according to polarity. TLC, HPTLC, UV, HPLC, IR, NMR and mass spectroscopy carried out. Tri-terpenoids Lupeol has been isolated from stem bark of Ziziphus xylopyrus, (Retz) Willd and their structure determined by spectral data chemical data. The results of the study can serve as a valuable source of information and provide suitable standards for identification of this plant material in future investigation and application. It was determined and isolated from the stem bark of Z. xylopyrus for the first time.
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